carboxypeptidasey from saccharomyces cerevisiae conformational difference reflected in kinetic behaviour in water and deuterium oxide

Carboxypeptidasey from Saccharomyces cerevisiae Conformational Difference Reflected in Kinetic Behaviour in Water and Deuterium Oxide

Efficiency of Saccharomyces Cerevisiae in Ceftriaxone Removal from Aquatic Environments: Kinetic, Isotherm of Absorption and Thermodynamics Study

Background & objectives: Antibiotics are potentially harmful contaminants, which can cause harmful effects on environmental equilibrium of ecosystems and the food chain. The aim of this study was to investigate the removal of ceftriaxone antibiotic by Saccharomyces Cerevisiae biosorbent from aquatic environments. Methods: This experimental study was conducted in a lab-scale batch reactor as dis...

Physicochemical and kinetic properties of acid phosphatase from Saccharomyces cerevisiae.

Acid phosphatase from yeast Saccharomyces cerevisiae was purified, and its physicochemical and kinetic properties were investigated. The sedimentation coefficient has been determined to be s0(20,w) = 13.6 S. The diffusion constant has been found to be 3.9 X 10(-7) cm2s-1, and the calculated partial specific volume was v = 0.663 cm3/g. From these data, a molecular weight of 252,000 was calculate...

CLONING AND EXPRESSION OF HUMAN IFNα2B GENE IN SACCHAROMYCES CEREVISIAE

Interferon is a protein secreted by eucaryotic cells following stimulation by viruses, bacteria, and many other immunogenes. Recent medical studies indicate that interferons have effective role in the treatment of virus infections, immunodeficiency and certain types of cancer such as hairy cell leukaemia (HCL). The aim of the present study is to apply yeast strain for secreting human IFNα2b fol...

Isolation, Subtype Determination, Cloning and Expression of HBsAg Gene from an Iranian Carrier in Saccharomyces cerevisiae

The Hepatitis B Surface antigen ( HBsAg) gene was isolated from an Iranian HBeAg positive carrier by PCR. The gene was cloned in pUC19 for sequencing and pYES2 for expression in Saccharomyces cerevisiae, which pNF1 and pDF3 constructs were made respectively. The sequencing data showed that the isolated HBsAg gene shared more than 90% homology with the ayw subtype. The pDF3 was transferred into ...

Water-Transfer Slows Aging in Saccharomyces cerevisiae.

Transferring Saccharomyces cerevisiae cells to water is known to extend their lifespan. However, it is unclear whether this lifespan extension is due to slowing the aging process or merely keeping old yeast alive. Here we show that in water-transferred yeast, the toxicity of polyQ proteins is decreased and the aging biomarker 47Q aggregates at a reduced rate and to a lesser extent. These benefi...